August 2nd, 2021

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Provoked Vulvodynia: Type of Lactobacilli Retrieved
Authors:  John Garza, Ph.D., Kushal Gandhi, Ph.D., Paula Gutierrez, M.S., Obianuju Okoye, MS3, Asley Sanchez, B.S., and Gary Ventolini, M.D.
  OBJECTIVE: Provoked vulvodynia is a chronic gynecologic condition affecting around 8–13% of the female population in the world. There are no evidence-based management recommendations for provoked vulvodynia, and only few randomized controlled trials have been performed. The objective of our study was to determine and compare Lactobacillus species collected from vaginal swabs of provoked vulvodynia and control patients.

STUDY DESIGN: Saline wet mount vaginal swabs were obtained from 40 patients: 20 patients clinically diagnosed with provoked vulvodynia and a control group of 20 patients. Lactobacilli were assessed through Gram classification and colony morphology methods, and vaginal Lactobacillus species were identified by a real-time polymerase chain reaction analysis.

RESULTS: Modified permutational multivariate analysis of variance using Bray-Curtis dissimilarity demonstrated a significant difference in Lactobacillus species composition between patients with provoked vulvodynia and controls (*p=0.008).

CONCLUSION: Lactobacillus gasseri was significantly less predominant in patients with provoked vulvodynia. Lactobacillus iners was a more frequently dominant species in patients with provoked vulvodynia. The colony morphology of the vaginal swab sample from the provoked vulvodynia group was dissimilar to that of the control group.
Keywords:  bacterial toxins; bacterial vaginosis; dysbiosis, microbiology; female; genome, bacterial; inflammation; lactobacilli; lactobacillus; microbiome; provoked vulvodynia; vagina, microbiology; vaginal dysbiosis; vaginal microbiota; vaginosis, bacterial; vulvodynia
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