August 2nd, 2021

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Concentrations of Angiogenic Factors in Relation to Anti-Müllerian Hormone in Human Preovulatory Follicular Fluid
Authors:  Akemi Nishigaki, Ph.D., Tomoko Tsuzuki-Nakao, M.D., Kayo Shimoi, B.D., Masahiro Yoshimura, B.D., Naoko Kida, M.D., and Hidetaka Okada, M.D.
  OBJECTIVE: To investigate the potential role of antiMüllerian hormone (AMH) in regulating angiogenesis during folliculogenesis by examining the relationship between AMH and angiogenic factors in individual human preovulatory follicles.

STUDY DESIGN: The study included 36 women undergoing invitro fertilization. Serum samples and follicular fluid (FF) were collected on the day of oocyte retrieval. Concentrations of AMH, vascular endothelial growth factor (VEGF), angiopoietin-1 (ANGPT1), and ANGPT2 in 438 follicular fluid samples were measured with enzyme-linked immunosorbent assay.

RESULTS: On average, the AMH concentration in the FF was ~1.5-fold greater than that in the serum. Moreover, the serum concentrations of AMH showed a positive correlation with the AMH concentrations in the FF. The concentration of VEGF in the FF significantly decreased with increasing AMH concentration in the FF. However, while VEGF levels in FF also decreased with increasing serum AMH levels, the decrease only reached the borderline of significance (p=0.08). The concentration of FF ANGPT2 significantly decreased with increasing FF and serum AMH concentrations, whereas that of FF ANGPT1 was unaltered with change in FF and serum AMH levels.

CONCLUSION: Our data suggest that AMH plays an important role in regulating angiogenic factors during folliculogenesis.
Keywords:  AMH receptor, angiogenic factor, angiopoietin-1, angiopoietin-2, anti-Müllerian hormone receptor, assisted reproductive techniques, follicular fluid, invitro fertilization, ovary, vascular endothelial growth factor
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