August 9th, 2022

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Four Single Nucleotide Polymorphisms in INSR, SLC6A14, TAS2R38, and OR2W3 Genes in Association with Idiopathic Infertility in Persian Men
Authors:  Elham Siasi, Ph.D., and Ahmad Aleyasin, Ph.D.
  OBJECTIVE: To analyze and evaluate 4 single nucleotide polymorphisms (SNPs)T132903C, C109869T, T824C, and T886Cand their correlation to the idiopathic Persian (Iranian) infertile male with oligospermia and azoospermia.

STUDY DESIGN: A total of 96 idiopathic infertile male patients and 100 normal fer-
tile men (controls) were included in the study. SNP analysis was performed using Real-Time High Resolution Melt analysis (PCR-HRM). Results were confirmed using PCR-RFLP and DNA sequencing analysis.

RESULTS: The frequency of 2 SNPs, T132903C in INSR and C109869T in SLC6A14, were statistically different in the infertile males as compared to the control males (p<0.02 for T132903C and p<0.04 for C109869T). The SNP frequency for T824C in OR2W3 and T886C in TAS2R38 were similar in the infertile men and the control group with p values of <0.2 for the T824C and p<0.9 for T886C SNPs, respectively.

CONCLUSION: Our results indicate a significant correlation between T132903C and C109869T SNPs in the INSR and SLC6A14 genes with idiopathic infertility in Persian males. These SNPs may also play a role in defects in spermatogenesis (oligospermia and azoospermia). Our study on Persian infertile male patients was surprisingly consistent with what others have reported for the European male population. Such similarity might indicate a solid and crucial involvement of these SNPs in idiopathic male infertility in general.
Keywords:  genes; high resolution melting (HRM) analysis; idiopathic male infertility; infertility, male; restriction fragment length polymorphisms; RFLP genotyping; single nucleotide polymorphism; SNPs
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