August 2nd, 2021

A full text version of this article is available.
To access article obtain online access here or login
Evaluation of IGF2 Imprinting Status and the Levels of Folate Biomarkers in Second Trimester Pregnant Chinese Women
Authors:  R.-F. Gao, M.D., J.-L. He, M.D., X.-Q. Liu, M.D., Y.-B. Ding, M.D., X.-M. Chen, Ph.D., Q. Chen, M.D., Y. Tan, M.D., and Y.-X. Wang, M.D.
  OBJECTIVE: To assess the relationship between folate biomarkers levels and IGF2 imprinting status among second trimester pregnant Chinese women.

STUDY DESIGN: Three hundred women in their second trimester were screened by polymerase chain reaction– restriction fragment length polymorphism (PCR-RFLP) for analysis of IGF2 imprinting status. Statistical differences of folate biomarkers levels were calculated in the groups with different imprinting status.

RESULTS: Of the 300 women analyzed, 133 (44.33%) cases were homozygous allele with A, 3 (1.00%) cases were homozygous allele with G, while 164 (54.67%) cases were heterozygous with A and G, qualifying them for analysis of loss of imprinting (LOI). Among the 164 cases undergoing LOI analysis, 44 (26.83%) were IGF2 LOI cases, while 120 (73.17%) were IGF2 retention of imprinting (ROI) ones. The mean level of serum folate, vitamin B12 and tHcy was 28.46±10.74 ng/mL, 380.20±206.13 pg/mL, 14.24±6.34 µmol/L among women with IGF2 ROI, and 30.89±9.97 ng/mL, 394.28±195.92 pg/ mL, and 13.12±6.23 µmol/L among women with IGF2 LOI, respectively.

CONCLUSION: No significant difference of folate biomarkers levels was observed between IGF2 ROI and IGF2 LOI groups.
Keywords:  folate, genomic imprinting, homocysteine, insulin-like growth factor 2, vitamin B12
  Acrobat Reader 7.0 is recommended to properly view and print the article.
Reader can be downloaded from