August 2nd, 2021

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Title:
Detection of Phosphatidylglycerol in Frozen Amniotic Fluid by Rapid Assay of Fetal Lung Maturity
Authors:  Craig V. Towers, M.D.
  OBJECTIVE: The study objective was to analyze the accuracy of the antibody-mediated rapid assay slide agglutination AmnioStat-FLM (Irvine Scientific, Santa Ana, CA) test in detecting the presence or absence of phosphatidylglycerol (PG) in amniotic fluid samples that have been frozen.

STUDY DESIGN: A prospective blinded evaluation was performed in which fresh samples of amniotic fluid were initially analyzed for PG by the rapid assay
(AmnioStat-FLM) test and the results reported for patient management. The specimens were then coded, patient identification was removed and specimens were then frozen. The samples were then thawed and the
AmnioStat-FLM test was performed a second time with the laboratory technicians blinded to the original test result. Neonatal outcome and the source of the sample, including the presence of blood and meconium, were recorded.

RESULTS: A total of 200 specimens were evaluated with 197 (98.5%) concordant between the initial test result and the result after the freeze-thaw process regarding the reporting results of either the presence or absence of PG (95% CI 94-99%). The result also was not affected by the source of the specimen or the presence of blood or meconium.

CONCLUSION: Freezing and thawing of amniotic fluid had minimal affect on the antibody-mediated rapid assay slide agglutination (AmnioStat-FLM) test result. (J Reprod Med 2008; 53:320-322)
Keywords:  amniotic fluid, fetal development, phosphatidylglycerol
   
   
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