August 23rd, 2019

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SMCP Gene and Protein Expression in Motive Sperm from Chinese Infertile Men with Asthenozoospermia
Authors:  Yushan Lee, M.S., Ph.D., Hetao Chen, M.S., Xiyang Yang, M.S., Junjie Liu, M.S., Linkai Wang, M.S., and Yanhua Su, M.S.
  OBJECTIVE: To evaluate whether the expression of sperm mitochondria-associated cysteine-rich protein (SMCP) gene, which has been reported to be highly associated with male infertility, is changed in the spermatozoa of Chinese asthenozoospermia patients.

STUDY DESIGN: A total of 50 infertile men with idiopathic asthenozoospermia attending a reproductive center, along with 50 fertile men, were selected for semen, sperm SMCP mRNA, and protein expression analyses using real-time reverse transcription-polymerase chain reaction and western blotting.

RESULTS: The mean levels of the sperm SMCP gene and protein were significantly decreased in infertile men with asthenozoospermia as compared to the normal controls. Sperm SMCP gene and protein expression demonstrated a significant positive correlation with sperm motility (r=0.716, p=0.001; r=0.705, p=0.001).

CONCLUSION: The expression of sperm SMCP was significantly decreased in an asthenozoospermic sample of the Chinese population, and sperm SMCP expression showed a significant positive correlation with sperm motility. SMCP may play an important role in the pathophysiology of male infertility.
Keywords:  assisted reproductive technologies; asthenozoospermia; male infertility; SMCP; sperm motility; real time RT-PCR; western blotting
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