February 15th, 2019

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Expression, Correlation, and Clinical Significance of DACH1 and ERα in Human Endometriosis Tissue
Authors:  Fangfang Lang, M.D., Xiaoxia Che, M.D., Zhenghui Fang, M.D., Zhe Li, M.D., Yang Weng, M.D., and Jie Jiang, Ph.D.
  OBJECTIVE: To study the expression, correlation, and clinical significance of DACH1 and ERα in human endometriosis tissue.

STUDY DESIGN: Reverse transcription polymerase chain reaction (RT-PCR), real-time PCR, and immunohistochemistry were used to assess DACH1 aand ERα mRNA expression levels in 51 cases of ovarian endometriosis, 11 cases of adenomyosis, 9 cases of ectopic peritoneal tissue, 17 cases of eutopic endometrium, and 30 cases of normal endometrium. A follow-up study of all patients’ relapse rates was conducted.

RESULTS: We found that the expression rates of DACH1 in ovarian ectopic endometrium (34/51, 66.7%), adenomyosis (6/11), and ectopic peritoneal tissue (6/9) were significantly lower than in normal endometrium (28/30, 93.3%) and eutopic endometrium (14/17; p< 0.05). The rate of ERα expression in ovarian ectopic endometrium (32/51, 62.7%), adenomyosis (5/11), and peritoneal ectopic tissue (5/9) were significantly lower than in normal endometrium (27/30, 90%). Furthermore, the expression rates of DACH1 and ERα mRNA in endometriosis were significantly lower than in the endometrial tissue (p<0.05). The data demonstrated that the expression of DACH1 and ERα are negatively correlated. Finally, we found that rate of recurrence for DACH1-positive patients was lower than that for DACH1-negative patients.

CONCLUSION: We confirm the findings that DACH1 is less frequently expressed in endometriosis than in normal endometrium and suppresses the function of ERα. These findings are important for the study and diagnosis of endometriosis occurrence and recurrence.
Keywords:  DACH1, Dachshund homolog 1, endometrioma, endometriosis, ERα, estrogen receptor alpha, gene expression profiling, mRNA differential display
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