December 17th, 2017

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Title:
Permissive Human Cytomegalovirus Infection of the First Trimester Cells at the Maternal-Fetal Interface
Authors:  Shu Zhou, M.D., Li-Qun Dong, M.D., Xiao-Mei Sun, M.D., Lin-Bo Guan, M.D., Jun Zhu, M.D., Li-Xing Yuan, M.D., and Ping Yu, Ph.D.
  OBJECTIVE: To explore the susceptibility to human cytomegalovirus (HCMV) of primary human cells and cell lines at the maternal-fetal interface.

STUDY DESIGN: Primary human early pregnancy extravillous trophoblasts, primary human villous fibroblasts, and primary human umbilical vein endothelial cells were isolated and purified, and then HCMV was inoculated with these primary cells and 3 cell lines including human extravillous trophoblast cell line (HTR8/Svneo), human normal lung fibroblast (MRC-5), and human umbilical vein endothelial cell line. Four detection methods specific for HCMV, in which PCR for HCMV DNA sequences, immunofluorescence for pp65 antigen, Western blot for HCMV gB protein, as well as cytopathic effect observation were conducted at different times postinfection.

RESULTS: All primary human cells and cell lines at the maternal-fetal interface were HCMV-positive, and the occurring time of cytopathic effect was different in these cells. HCMV DNA was also detected in cell culture supernatant, indicating HCMV replication and the release of virus.

CONCLUSION: The first trimester cells at the maternal-fetal interface are fully permissive for HCMV infection.
Keywords:  human cytomegalovirus; infection; trophoblasts; umbilical vein endothelial cells; villous fibroblasts
   
   
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