March 23rd, 2018

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Circadian Gene Expression in Ovarian Follicles of Egg Donors Undergoing Controlled Ovarian Hyperstimulation
Authors:  Edward D. Tarnawa, M.D., John A. Schnorr, M.D., John Baatz, Ph.D., and Christopher J. Robinson, M.D., MSCR
  OBJECTIVE: To identify circadian gene expression in human granulosa cells and confirm synthesis of corresponding circadian proteins.
METHODS: Granulosa cells were isolated from 12 oocyte donors undergoing controlled ovarian hyperstimulation. Real-time PCR was used to investigate expression profiles for 5 core circadian genes. Western blotting was used to detect Clock and Per1 proteins.

RESULTS: Circadian genes are expressed in human granulosa cells (average Ct value 23.5–27.1). Clock and Per1 proteins were detected at varying intensities among patient samples normalized by total protein.
CONCLUSIONS: Circadian genes are expressed in human granulosa cells, and at levels higher than expected. Furthermore, circadian proteins are synthesized and presumed to play a role in the function of the human ovarian follicle.
Keywords:  circadian clock proteins and peptides, circadian gene expression, circadian rhythm, circadian rhythm signaling peptides, circadian rhythm signaling proteins, clock genes, CLOCK proteins, human, granulosa cell, ovary, suprachiasmatic nucleus
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