August 24th, 2017

Next
A full text version of this article is available.
To access article obtain online access here or login
 
Title:
Circadian Gene Expression in Ovarian Follicles of Egg Donors Undergoing Controlled Ovarian Hyperstimulation
Authors:  Edward D. Tarnawa, M.D., John A. Schnorr, M.D., John Baatz, Ph.D., and Christopher J. Robinson, M.D., MSCR
  OBJECTIVE: To identify circadian gene expression in human granulosa cells and confirm synthesis of corresponding circadian proteins.
METHODS: Granulosa cells were isolated from 12 oocyte donors undergoing controlled ovarian hyperstimulation. Real-time PCR was used to investigate expression profiles for 5 core circadian genes. Western blotting was used to detect Clock and Per1 proteins.

RESULTS: Circadian genes are expressed in human granulosa cells (average Ct value 23.5–27.1). Clock and Per1 proteins were detected at varying intensities among patient samples normalized by total protein.
CONCLUSIONS: Circadian genes are expressed in human granulosa cells, and at levels higher than expected. Furthermore, circadian proteins are synthesized and presumed to play a role in the function of the human ovarian follicle.
Keywords:  circadian clock proteins and peptides, circadian gene expression, circadian rhythm, circadian rhythm signaling peptides, circadian rhythm signaling proteins, clock genes, CLOCK proteins, human, granulosa cell, ovary, suprachiasmatic nucleus
   
   
  Acrobat Reader 7.0 is recommended to properly view and print the article.
Reader can be downloaded from