July 26th, 2017

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Title:
Low Expression of LEFTY1 in Placental Villi Is Associated with Early Unexplained Miscarriage
Authors:  Huiqin Xue, M.D., Lan Ma, M.B.B.S., Jinjie Xue, M.D., Xiayu Sun, M.B.B.S., Ming Li, M.B.B.S., Hongyong Lu, M.M., Yan Zhou, M.B.B.S., Yuezhen Guo, M.B.B.S., Yuping Zhang, M.M., and Jigeng Bai, M.M.
  OBJECTIVE: To investigate the function and underlying mechanism of transforming growth factor–beta (TGF-β)/bone morphogenetic protein (BMP) signaling pathway in early unexplained miscarriage.

STUDY DESIGN: Expression profiles of genes involved in TGF-β/BMP signaling pathway were compared between placental villous tissue samples from 2 women with missed abortion and those from 2 women with induced abortion by microarray assay. The protein expression level of the most downregulated gene—LEFTY1—was further measured using western blotting in another 8 women with missed abortion and 7 women with induced abortion.

RESULTS: A total of 24 genes showed differential expression level between the 2 groups. Their functions were further investigated, of which 6 of 13 upregulated genes were TGF-β responsive genes. The most reduced gene is LEFTY1, an antagonist of TGF-β ligand. The protein expression level of LEFTY1 was confirmed to show the same trend as microarray using western blotting.

CONCLUSION: A reduced expression of LEFTY1 in women with missed abortion was identified as com-pared with women with induced abortion, which may result in a dysregulation of TGF-β signaling and may be the underlying mechanism of missed abortion.
Keywords:  abortion, missed; BMP type I receptor; bone morphogenetic protein receptor type I; chorionic villi; gene expression profiling; LEFTY1 protein, human; placental villi; receptors, transforming growth factor beta; TGF β/BMP signaling pathway; TGF-beta; TGF-beta receptors; TGF-beta superfamily proteins; transforming growth factor beta receptor
   
   
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