April 8th, 2020

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Antiadhesive Role of Human Amniotic Fluid on Peritoneal Adhesion Formation in a Rat Model: Experimental Study
Authors:  Deniz Hizli, M.D., Saynur Yilmaz, M.D., Nermin Kosus, M.D., Aydin Kosus, M.D., Hacer Haltas, M.D., Fatih Hizli, M.D., and Hasan Kafali, M.D.
  OBJECTIVE: To determine the role of human amniotic fluid (HAF) in preventing or reducing postoperative adhesions.

STUDY DESIGN: Uterine horn adhesion model was carried out in 24 female Wistar rats. The animals were randomized into 4 groups: (1) control, (2) Ringerís lactate, (3) whole HAF, and (4) HAF depleted from cells and proteins. Adhesion grade and histologic findings of adhesion-carrying tissues were evaluated and groups were compared according to these parameters.

RESULTS: Rats treated with whole HAF had less adhesion grade when compared to the control group, but the difference was not statistically significant. On the other hand, centrifuged amniotic fluid treatment significantly reduced peritoneal adhesion grade, fibrosis and inflammation (p<0.05).

CONCLUSION: Whole HAF seems to have no beneficial effect on peritoneal adhesion formation, but HAF depleted of protein and cells does have a positive effect on reducing adhesion formation.
Keywords:  amniotic fluid; centrifugation; surgical adhesions
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